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Arsenic represses β-catenin expression in the differentiating EBs. P19 cells were aggregated for 2 and 5 days with (A and C) or without (B and D) 0.5μM sodium arsenite. EBs were harvested and embedded with paraffin, and β-catenin expression was examined by immunofluorescence. Pictures are representative examples from 198 EBs per time point per group.
