Fig. 3.

Effect of iron overload on NRF2 downstream proteins in TMHF-fed mice. (A) Table showing NRF2-regulated enzymes that fell into the four categories. Upon binding to ARE, NRF2 transactivates genes associated with phase I/II drug metabolism, GSH synthesis, antioxidants, and NADPH regeneration. Numbers in parenthesis are fold increase from iTRAQ profiling. (B–E) Liver protein from mice fed a control or TMHF diet for 4 weeks was subjected to immunoblotting to verify and extend identification of NRF2-regulated proteins associated with (B) phase I/II drug metabolism, (C) GSH synthesis, (D) antioxidants, and (E) NADPH regeneration and increased in level of expression by TMHF treatment.