Fig. 4.

Translocation of NRF2 to the nucleus in livers from TMHF-treated mice. (A) Liver cryosections from control and TMHF-fed mice were fixed and subjected to indirect immunofluorescence to detect NRF2 (green). Sections were mounted in Prolong Gold containing 4′,6-diamidino-2-phenylindole for nuclear staining (blue). Representative images of each stain alone and merged are shown. (B) Proposed schematic of iron-induced activation of NRF2. Oxidative stress produced by overload iron causes generation of ROS, which triggers release of NRF2 from Keap1. NRF2 levels are increased. NRF2 translocates to the nucleus and binds to the ARE of NRF2 target genes activating phase I/II and antioxidant gene transcription.