Table 2.
Bestkeeper correlation and regression analysis of housekeeping genes
| 18S rRNA | GAPDH | ACTB | ATP5B/ ATP5G1cp | |
|---|---|---|---|---|
| |
vs. |
vs. |
vs. |
vs. |
| |
BestKeeper |
BestKeeper |
BestKeeper |
BestKeeper |
| |
Coefficient of correlation [r] |
|||
|
HBECs |
0.962 |
0.871 |
0.709 |
0.543 |
|
PTECs |
0.843 |
0.743 |
0.741 |
0.73 |
|
Chicken lung cells |
0.944 |
0.832 |
0.845 |
- |
| Duck lung cells | 0.836 | 0.792 | 0.542 | - |
Pairwise correlation and regression analysis using Bestkeeper correlation analysis (n=12) found 18S rRNA as the most suitable housekeeping gene. Note that highest correlation between 18S rRNA and the BestKeeper index (p<0.01) was found in all the four cell types used. *ATP5B and ATP5G1were included in HBECs and PTECs analysis respectively.