Figure 4.

Quantitative immunodetection analysis of PSII and PSI antenna (Lhcb1, Lhca1) and core (PsbA, PsaA) proteins. Pea and bean thylakoid samples of increasing amount of chlorophyll starting from 0.25 or 0.5 to 2 or 4 μg for antenna and core proteins, respectively were separated by SDS-PAGE. Pea/bean ratios are calculated on the basis of pixel intensity analysis and are mean values ± SD for three to five separate experiments. The presented Chl a/Chl b ratios are mean value ± SD for at least fifteen independent measurements. Pea/bean ratio for Lhca1 protein was determined for main (*) or all (**) detected bands.