Figure 6. D2-like signaling promotes mating fitness.

(A) Y-axis depicts the number of pregnant females amongst 1, 5 or 20 potential mates. Symbols represent individual male's sexual prowess. (B) Mating competition test pairing a wild type and a mutant male with one fog-2 female +/−10 paralyzed males (first 4 columns), and number of fog-2 females impregnated when wild type and dop-2; dop-3 males were paired individually with 10 C. briggsae hermaphrodites ( last 2 columns). The top and bottom of each column indicates the % of pregnant females and the number of males that copulated or number of males assayed (last 2 columns). (C) The cumulative time in contact with males that a wild type and dop-2; dop-3 male, represented by each symbol, spent when surrounded by 40–50 paralyzed males. Each data subset represents non-ablated animals (mock), PCB or PCS ablated animals. p-values calculated using the Mann-Whitney test. For A&C, line and error bars represent mean and SEM. (D) Top shows coincident D2-like and ACh signaling during spicule insertion attempts. Bottom depicts residual DA signaling when a male moves off the vulva. Blue and green color represents DA and ACh signaling components, respectively.