. 2012 Sep 1;4(5):578–585. doi: 10.4161/mabs.21328

Table 3. Impact of deamidation.

Locations	Effects	Reference
Light chain, CDR1	Asp30/Asn30 only 70% as potent as Asn/Asn30	²
Light chain CDR1	Asp30/Asn30 has 39% and isoAsp30/Asn30 has 60% of binding affinity compared with Asn30/Asn30 of 96% binding affinity. Slight difference in the secondary structure of Fab with isoAsp compared with Fab with Asn30. Higher Tm for Fab with Asp30 and lower Tm for Fab with isoAsp30 compared with Fab with Asn30	²⁷
Heavy chain CDR2	14 fold reduction in binding affinity compared with the antibody with Asn	²⁸
Heavy chain CDR2	IsoAsp55 or Asp55 in one heavy chain only 20% potent as antibody with two Asn. Fab with either isoAsp55 or Asp55 has only 50% binding affinity compared with Fab with Asn.	²³
Heavy chain CDR2	No impact on potency for fraction with lower level of Asn55 deamidation	17