Figure 3.
Ten micrograms of total RNA from young leaves (a) and sclerenchyma tissue (b) of control (lanes C) and transgenic aspen (lanes S10–S14) was probed with 32P-labeled CAOMT cDNA. The rRNA was visualized with UV illumination as a control for the RNA-loading amount. Ten micrograms of total RNA from the xylem of control (lanes C) and various transgenic aspen lines (lanes S3–S14) was subjected to primer-extension analysis using a CAOMT gene-specific oligonucleotide (c) and a 4CL gene-specific oligonucleotide (d) as a control. The extension products of the endogenous CAOMT transcript are located 124 nucleotides (nt) upstream from the primer and the expected size of the extension products of transgene mRNA is indicated by the arrowhead.