Table I.
Biochemical and chemical characterization of control (C) and transgenic aspen
| Plant No. | Lignin Composition
|
Xylem
CAOMT Activity
|
Wood Colorationa | |||||
|---|---|---|---|---|---|---|---|---|
| G | 5-OH G | S | S/G | CA | 5HFA | 5HFA/CA | ||
| μmol g−1 Klason lignin | pkat mg−1 protein | |||||||
| C | 700 ± 33 | – | 1485 ± 106 | 2.12 | 114.6 ± 7.2 | 265.0 ± 7.8 | 2.3 | −a |
| S10 | 757 ± 28 | – | 1323 ± 44 | 1.75 | 144.0 ± 2.5 | 261.2 ± 11.0 | 1.8 | +b |
| S7 | 651 ± 18 | – | 1089 ± 29 | 1.67 | 136.1 ± 1.6 | 311.9 ± 26.1 | 2.3 | + |
| S12 | 706 ± 12 | – | 1166 ± 43 | 1.65 | 123.3 ± 9.2 | 220.7 ± 5.5 | 1.8 | ++c |
| S14 | 938 ± 42 | – | 1355 ± 49 | 1.44 | 101.8 ± 3.5 | 210.9 ± 4.6 | 2.1 | +++d |
| S3 | 875 ± 16 | – | 1360 ± 15 | 1.55 | 78.2 ± 3.1 | 180.7 ± 14.3 | 2.3 | +++ |
| S13 | 905 ± 29 | 118 ± 4 | 506 ± 17 | 0.56 | 32.5 ± 0.7 | 61.2 ± 0.7 | 1.9 | ++++e |
5-OH G, 5-Hydroxyguaiacyl unit; CA, caffeic acid; 5HFA, 5-hydroxyferulic acid.
a
−, No coloration.
b
+, Slightly colored.
c
++, Moderately colored.
d
+++, Highly colored.
e
++++, Entirely colored.