Figure 2. Characterization of YP7 binding properties. (A) Flow cytometry analysis using a panel of endogenous GPC3–expressed human cell lines. Four HCC (HepG2, Hep3B, Huh4, Huh7), four CCA (HuCCT1, KMBC, KMCH and Mz-ChA-1), one ovarian CCC (TOV-21G) and one melanoma (UACC-62) cell lines were shown. Filled peak: isotype control. (B) Binding affinity measurement of YP7 against cell surface-associated GPC3. MFI: mean fluorescence intensity. The Scatchard plot and the KD value were calculated using Prism. (C) Binding of YP7 on recombinant GPC1, GPC3 and GPC5 proteins.