Figure 1. Generation of ADA-a2H bispecific Zybody. (A) Schematic diagram to illustrate ADA-a2H generated by fusion of Ang2 specific peptide to C-terminus H-chains of the adalimumab scaffold; (B) Size exclusion chromatography (SEC) profiles of adalimumab; solid line-ADA-a2H, dotted line- adalimumab; (C) Effect of storage at 30°C on the monomer purity of adalimumab and ADA-a2H as measured by SEC; (D) SDS PAGE analysis of purified ADA-a2H (lane 2 and 4) and adalimumab (lane 1 and 3); (E) DSC analysis of ADA-a2H and adalimumab.