Figure 3. Influence of irradiation on motiliy, measured by scratch assay. (A) Representative images of cells directly (0 h) or 10 h after scratch in controls (con) or cells irradiated with 10 Gy (X-ray). Upper panel: cells 1 d after irradiation, lower panel: cells 7 d after irradiation. (B) Measured scratch widths. Upper panel: cells were seeded, irradiated with 5 or 10 Gy or remained untreated, cultured overnight and the scratch was generated. Scratch width at five sites on a particular position was measured after 4, 6, 8, 10 and 24 h. Lower panel: cells were irradiated with 5 or 10 Gy and cultured for another 6 d. Treated and untreated cells were seeded and cultured overnight before starting with the assay as described above. Three independent experiments were performed. Symbols represent means ± SEM, (p ≤ 0.05), *, vs. 5 and 10 Gy; #, vs. 5 Gy; §, vs. 10 Gy. ANOVA, Bonferroni post hoc method.