Figure 6. Representative western blots showing EphA2 and EphA3 receptor in untreated or irradiated melanoma cell lines. Cells were lysed, followed by protein quantification using BCA protein assay. Protein extracts were subjected to SDS-PAGE, western blotting and immunodetection using specific antibodies. A polyclonal actin antibody was used to verify equal protein amount. For each cell line at least three independent protein samples were analyzed. (A) EphA2 and EphA3 receptor in untreated melanoma cells. (B) EphA2 and EphA3 receptor in irradiated (5 and 10 Gy, 1 d and 7 d after X-ray) melanoma cells. (C) EphA2 receptor was immunoprecipitated from protein lysates from A375 cells. Immunoprecipitates were subjected to SDS-PAGE, western blotting and immunodetection using specific antibody to phosphorylated tyrosine and reprobed with EphA2 antibody.