Figure 7. Representative western blots showing Src and FAK phosphorylation in untreated or irradiated melanoma cell lines. Cells were lysed, followed by protein quantification using BCA protein assay. Protein extracts were subjected to SDS-PAGE, western blotting and immunodetection using specific antibodies. Immundetection of the phospho-specific antibodies was followed by stripping and reprobing with anti-Src or anti-FAK. A polyclonal actin antibody was used to verify equal protein amount. For each cell line at least three independent protein samples were analyzed. (A) P-Src and Src kinase in untreated melanoma cells. (B) P-FAK and FAK in untreated melanoma cells. (C) P-Src/Src and P-FAK/FAK in irradiated (5 and 10 Gy, 1 d and 7 d after X-ray) melanoma cells.