Table 1. Expression of selected Eph receptors and ephrin ligands as determined by RT-PCR.
| Transcript name | Mel-Juso | Mel-Juso-L3 | A375 | A2058 |
|---|---|---|---|---|
| EphA1 |
- |
- |
+ |
- |
| EphA2 |
+ |
+ |
+ |
+ |
| EphA3 |
+ |
+ |
+ |
+ |
| EphA4 |
+ |
+ |
+ |
+ |
| EphA5 |
+ |
+ |
- |
+ |
| EphA7 |
+ |
- |
+ |
- |
| EphB1 |
+ |
+ |
+ |
- |
| EphB2 |
+ |
+ |
+ |
+ |
| EphB3 |
+ |
+ |
+ |
+ |
| EphB4 |
+ |
+ |
+ |
+ |
| EphB6 |
- |
- |
- |
- |
| ephrinA1 |
- |
- |
+ |
+ |
| ephrinA2 |
+ |
+ |
+ |
+ |
| ephrinA3 |
- |
+ |
+ |
+ |
| ephrinA5 |
+ |
+ |
+ |
+ |
| ephrinB1 |
+ |
+ |
+ |
+ |
| ephrinB2 | + | + | + | + |
+, proved mRNA expression; -, no mRNA expression detectable. Human endothelial cells from umbilical vein (HUVEC) or aorta (HAEC) were used as positive controls to confirm accuracy of RT-PCR reaction (see Methods section).