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. 2012 Mar 1;6(2):113–125. doi: 10.4161/cam.20655

Table 1. Expression of selected Eph receptors and ephrin ligands as determined by RT-PCR.

Transcript name Mel-Juso Mel-Juso-L3 A375 A2058
EphA1
-
-
+
-
EphA2
+
+
+
+
EphA3
+
+
+
+
EphA4
+
+
+
+
EphA5
+
+
-
+
EphA7
+
-
+
-
EphB1
+
+
+
-
EphB2
+
+
+
+
EphB3
+
+
+
+
EphB4
+
+
+
+
EphB6
-
-
-
-
ephrinA1
-
-
+
+
ephrinA2
+
+
+
+
ephrinA3
-
+
+
+
ephrinA5
+
+
+
+
ephrinB1
+
+
+
+
ephrinB2 + + + +

+, proved mRNA expression; -, no mRNA expression detectable. Human endothelial cells from umbilical vein (HUVEC) or aorta (HAEC) were used as positive controls to confirm accuracy of RT-PCR reaction (see Methods section).