Table 2. Primer sequences, annealing temperatures, expected sizes of amplified products and number of PCR cycles used for RT-PCR. Primers for transcripts of EphA2, EphA3, ephrinA1, ephrinA5 and 18S rRNA were further used for quantitative real-time RT-PCR.

Transcript name	NCBI accession number	Forward primer sequence (5′→3′)	Reverse primer sequence (5′→3′)	AT (°C)	PS (bp)	CN
EphA1‡	NM_005232	TGCAAGGTGTCTGACTTTGG	TCATCTCCCCATAAGGCTTG	58	205	32
EphA2*	NM_004431.2	GAAGAGCCCCGTATGCACT	GGCTCTCAGATGCCTCAAAC	60	145	36
EphA3*	NM_005233.5	GCTTGTACCCATTGGCAAGT	GCCAACTCCAGTCCAGGATA	58	293	28
EphA4*	NM_004438.3	GTCCACTCACAGTCCGCAATC	GCAAGCTTGGCATTCTCCGCT	66	217	28
EphA5†	NM_004439.4	CCTTCTGTGGTACGACACTTG	GGTCTGCACACTTGACAGGTG	60	221	36
EphA7‡	NM_004440	AAGCAGGCTACCAGCAAAAA	GGTCAGATGGAGCCCTGTAA	58	171	32
EphB1*	NM_004441.4	ACTCTACTGCAACGGGGATG	CCACTTCTGGAGGGTCAAAG	62	242	32
EphB2*	NM_017449	TCCATCTGGGACTTTCAAGG	GCATGAGGGAGGTCTCATTG	58	209	32
EphB3*	NM_004443.3	TCTGCACCTGCCACAATAAC	TGGCACTTCTTGCAGATGAC	60	193	36
EphB4†	NM_004444.4	TTCGGCCAGGAACATCACAG	CCGATGAGATACTGTCCGTG	58	200	32
EphB6†	NM_004445.2	GTTCTGGACGACCAGCGACG	GACGTTCAGTTGCAGTCCAG	60	408	36
ephrinA1†	NM_004428.2	CGGAATGAGGACTACACCATACATGTGCAGC	AAGCAGCGGTGTTGATGCTGGTGGATGGGTT	58	326	32
ephrinA2†	NM_001405.2	CTACACGGTGGAGGTGAGCA	ACAGCATTGGGAGGCGTGGCA	58	260	36
ephrinA3†	NM_004952.3	GCAGGTGAACGTGAACGACTATCT	AACTCGTAGCCCAGAGAGAAGGCGCT	60	261	36
ephrinA5†	NM_001962.1	CCAGCCGATAAGACTGAGCGC	CCATTATCTGGGATTGCAGAGG	58	230	32
ephrinB1†	NM_004429.3	CCAATGCTGTGACGCCTGAG	CGAACAATGCCACCTTGGAGTTG	58	222	36
ephrinB2†	NM_004093.2	GGAAGAAGTTCGACAACAAGTCC	TTCAGCAAGAGGACCACCAGTGT	58	182	32
18S rRNA§	NR_003286	CGGCTACCACATCCAAGGAAG	GCTGGAATTACCGCGGCTGCT	60	188

Source of primer sequences: *self-designed using “Primer3” program (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi). ^†Primers were designed as published by Fox et al.^{66 ‡}Primers were designed as published by Clifford et al.^{45 §}Primers were designed as published by Haase et al.⁶⁷ AT, annealing temperature; PS, product size; CN, number of PCR cycles.