Table 1. Assays used for quantitative methylation-specific polymerase chain reaction (qMSP).
| Assay | Sense primer | Antisense primer | Probe | Frg. size (bp) |
|---|---|---|---|---|
| ALU qMSP* |
GGTTAGGTATAGTGGTTTATATTTGTAATTTTAGTA |
ATTAACTAAACTAATCTTAAACTCCTAACCTCA |
6FAM-CCTACCTTAACCTCCC-MGB |
98 |
| CDO1 qMSP |
CGAATTATAGCGGCGGAGGT |
AAATCGCGTAAACTCCGCG |
6FAM-CGTTAGGTCGGGCGGT-MGB |
101 |
| DCLK1 qMSP |
GCGCGTACGCGGAGG |
CGACGACGAACGCGCT |
6FAM-CGGGAGGGCGTGTGA-MGB |
86 |
| SFRP1 qMSP# |
GAATTCGTTCGCGAGGGA |
AAACGAACCGCACTCGTTACC |
6FAM-CGTCACCGACGCGAA-MGB |
70 |
| ZSCAN18 qMSP | CGCGGTATAGTTTCGCGGTAT | CGCGATAACGACCGACAAA | 6FAM-CGTAGTTCGCGGTGAGG-MGB | 84 |