Figure 1. Heavy and Light chains variable region amplification. Total RNA of 8 healthy donors was extracted from peripheral blood mononuclear cells and cDNA was produced using oligo-dT. The cDNA was used to amplify heavy and light chains variable regions by PCR using the primers from Table 1. The figure shows representative PCR products from a single donor, resolved by electrophoresis on a 1% w/v agarose gel. MW: 100bp DNA ladder.