Figure 4. Sequence diversity of scFv obtained by ISA-PCR Plasmids were obtained from 14 random clones from the phage display library constructed using the scFv gene produced by ISA-PCR. Top panel shows the scFv genes produced by PCR resolved by electrophoresis on a 1% w/v agarose gel. Low panel shows digested scFv gene products with MvaI enzyme at 37°C for 3 h, resolved by electrophoresis on a 2% w/v agarose gel. $ and # indicates similar pattern. MW: 100bp DNA ladder.