Table 2. Results of binding selection using different targets.
| Antigen | Number of clones after 1st round of panninga | Number of clones after 2nd round of panninga | Number of binder clones after 2nd round of panninga | Number of different scFv |
|---|---|---|---|---|
|
Bovine serum albumin |
3.0x102 |
1.8x103 |
1/15 |
1/1 |
|
Human ErbB3 ectodomain |
2.3x104 |
3.7 x 106 |
3/12 |
3/3 |
|
Human tumor necrosis factor |
3.0x102 |
3.0x103 |
2/15 |
2/2 |
|
Human vimentin |
6.0x102 |
2.4x103 |
3/15 |
1/3 |
|
Human MHC class I-related chain A(MICA) |
6.6x103 |
1.0 x104 |
6/15 |
3/6 |
| Rotavirus | 6.0x102 | 1.5 x103 | 2/15 | 1/2 |
a Phage titer obtained after subtraction of the unspecific phage retrieved in controls.