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. 2012 Nov 15;8(11):e1003072. doi: 10.1371/journal.pgen.1003072

Figure 2. Ligand-recognition rules of AncSR1 and AncSR2.

Figure 2

A, The sensitivity of AncSR1-LBD (top panel) and AncSR2-LBD (bottom panel) to various hormones (Table S4) was characterized in a triplicate luciferase reporter assay and is displayed as EC50, the concentration at which half-maximal reporter activation is achieved. Error bars, 95% confidence interval. Sets of hormones are grouped by color and are numerically labeled according to the list below. B, AncSR1's ligand recognition criteria. Each pair of bars shows the EC50 of AncSR1 to a pair of hormones that differ only by aromatization of the A-ring (shown in red on the ligand structure and in the key). Unlike aromatization, substitution of a 17-keto or acetyl for estradiol's hydroxyl has only a weak effect on sensitivity, as shown by the small differences among pairs. C-I, AncSR2's ligand recognition criteria. Each pair of bars shows the sensitivity of the receptor to hormones that differ only in the functional group at specified positions or aromatization of the A-ring. Bar labels indicate the substance tested: 0, cholesterol, 1, 11-deoxycorticosterone, 2, 11-deoxycortisol; 3, corticosterone; 4, cortisol; 5, aldosterone; 6, progesterone; 7, 17α-hydroxyprogesterone; 8, 19-norprogesterone; 9, 4-pregnenolone; 10, 5-pregnenolone; 11, 20α hydroxyprogesterone; 12, 20β hydroxyprogesterone; 13, testosterone; 14, dihydrotestosterone; 15, 4-androstenediol; 16, 5-androstenediol; 17, 19-nortestosterone; 18, bolandiol; 19, estradiol; 20, estrone; 21, estriol; 22, 4-androstenedione; 23, 19-nor-1, 3, 5(10)-pregnatriene-3-ol-20-one (NPT).