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. 2012 Sep 11;11:66. doi: 10.1186/1476-4598-11-66

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Copyright ©2012 Gupta et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Western analyses in prostatic normal and tumor lysates. Total cellular (A and C), membrane (B and D) and nuclear (E to I) lysates from normal (NT) and prostatic tumor (TT) tissue (~20 μg protein) were immunoblotted (IB) with a RANKL (A and B), RUNX2 (E), phosphoserine (p-Serine; F), phospho-Smad 5 (p-Smad 5; G) and Smad 5 (H) antibody. Equal loading of the protein was shown in total cellular, membrane and nuclear lysates by relevant immunoblotting analysis with antibodies to GAPDH (C), actin (D) and nucleoporin (I). The results shown are representative of three independent experiments with three different lysates purchased from the vendor.