Figure 2. Proteolysis of free EgFABP1 and EgFABP1 bound to ligands.

Separation by SDS-PAGE of proteolytic fragments of apo-EgFABP1 (lane 1) and samples of this protein preincubated for 30 min with myristic acid (lane 2), palmitic acid (lane 3), stearic acid (lane 4) or oleic acid (lane 5) to obtain holo-forms, as indicated in Materials and Methods. Lane 6 corresponds to undigested EgFABP1. Samples were taken after 5 min (A) or 16 hours of proteolysis (B). A peptide molecular weight marker is shown in lanes labelled as MWM. This is a representative experiment from two independent experiments.