Figure 8. Inhibition of cytochrome c binding to anionic membrane by EgFABP1.

Addition of increasing amounts of cytochrome c to 15 µM SUVs containing 64% EPC, 10% EPE, 25% CL and 1% DPE caused a decrease in dansyl fluorescence emission. DPE-containing vesicles were >70% quenched upon addition of 1 µM cytochrome c in absence of EgFABP1 (inset). For the inhibition of binding analysis, 15 µM SUVs were incubated with increasing concentrations of EgFABP1 (0–48 µM), 1 µM cytochrome c was subsequently added, and the relative increase in dansyl fluorescence emission at 520 nm was monitored. Results are expressed as the percent relative fluorescence intensity, where 100% represents the relative fluorescence intensity of SUVs incubated in the presence of cytochrome c, but without EgFABP1. Results are the average of two experiments ± SD.