Figure 5. High-throughput sequencing of transposon integration sites in HEK-293 cells.

(a) Integration frequency near selected genomic features. Integration site data sets for each transposon are indicated by the columns and the distance from the integration site to the genomic features by the rows. The departure from random distribution is indicated by colored tiles. Blue indicates that insertions are depleted compared to random whereas red indicates features where insertions are enriched compared to random and gray indicates that the distribution is random. Differences from random placement were scored using the ROC area method. (b) Integration frequency of Sleeping Beauty, piggyBac, and TcBuster near transcription start sites. Integration sites near transcription start sites were compiled onto a common transcription start site and the proportions mapped. The x-axis shows the distance from the transcription start sites and the y-axis shows the percentage of integration sites that were found for each range of distances.