Table 2. Most highly abundant transcripts (contigs with >1,000 reads).
Contig | No. reads | Gh | Gb | 10 dpa | 22 dpa | Acc. Number* | Annotation |
step1_rep_c47611 | 2,355 | 1,294 | 1,061 | 1,065 | 1,290 | AAO92753.1 | GhH6 (arabinogalactan protein 2) |
step1_rep_c47637 | 2,242 | 894 | 1,348 | 706 | 1,536 | ADB54351.2 | cytochrome c oxidase |
step1_rep_c47617 | 1,971 | 1,088 | 883 | 1,193 | 778 | AAB03081.1 | E6 (protein kinase) |
step1_rep_c47607 | 1,363 | 335 | 1,028 | 0 | 1,363 | AAA84881.1 | FbLate-2** |
step1_rep_c47612 | 1,357 | 859 | 498 | 1,019 | 338 | AAL67991.1 | dehydration-induced protein RD22-like protein |
step1_rep_c47610 | 1,286 | 732 | 554 | 543 | 743 | ABO47740.1 | alpha-tubulin |
step1_rep_c47608 | 1,221 | 502 | 719 | 940 | 281 | AAG29777.1 | lipid transfer protein 3 precursor |
Contig_24629_bb | 1,147 | 801 | 346 | 703 | 444 | AAO18731.1 | cysteine protease |
step1_rep_c47623 | 1,096 | 349 | 747 | 420 | 676 | ABO47736.1 | alpha-tubulin |
step1_rep_c47619 | 1,043 | 583 | 460 | 838 | 205 | ABL86680.1 | alpha-expansin |
Blastx were based on nr protein database; all accessions were from Gossypium species as ranked among 5 best hit scores except ADB54351 from Allium.
the blastx annotation as FbLate-2 in G. barbadense ranked 11th (others were hypothetical protein), had 100% identity but only 25% coverage.
Number of reads of genotypes and fiber development stages are summed over 2 libraries each (Gh = Gh10+Gh22, Gb = Gb10+Gb22, 10 dpa = Gh10+Gb10, and 22 dpa = Gh22+Gb22).