Table 3. Pre-miRNAs identified in E. uniflora with sequence similarities to plant conserved miRNA families.

miRNA	Precursor miRNA						Mature miRNA
	Contig code	Length	GC %	MFE	AMFE	MFEI	5p sequence more abundant	Read count	3p more abundant sequence	Read count	Total reads
eun-MIR156	Contig92889	97	54.64	−55.20	−56.91	−1.04	TTGACAGAAGATAGAGAGCAC	83933	GCTCTCCCTCTCCTGTCAACA	1	85396
eun-MIR159	Contig93245	163	45.40	−55.86	−34.27	−0.75	AGCTGCTGGTCTATGGATCCC	376	CTTGCATATGCCAGGAGCTTC	493	1302
eun-MIR160	Contig81816	116	53.45	−54.30	−46.81	−0.88	TGCCTGGCTCCCTGTATGCCA	293	GCGTATGAGGAGCCAAGCATA	22	323
eun-MIR162	Contig165400	108	48.15	−35.80	−33.15	−0.69	GGAGGCAGCGGTTCATCGATC	24	TCGATAAACCTCTGCATCCAG	10713	10884
eun-MIR166	Contig94223	228	43.42	−72.40	−31.75	−0.73	GGAATGTTGTCTGGCTCGAGG	11016	TCGGACCAGGCTTCATTCCCC	381733	409546
eun-MIR167-1	Contig126350	90	45.56	−50.00	−55.56	−1.22	TGAAGCTGCCAGCATGATCTGA	616862	AGATCATCTGGCAGTTTCAAC	262	620306
eun-MIR167-2	Contig163487	615	37.40	−163.60	−26.60	−0.71	TGAAGCTGCCAGCATGATCTGG	32188	TCAGGTCATCTTGCAGCTTCA	939	34461
eun-MIR167-3	Contig784s	81	48.15	−38.60	−47.65	−0.99	TGAAGCTGCCAGCGTGATCTCA	16305	ATCAGATCATGTGGCAGCTTCACC	73	22056
eun-MIR1674	Contig784a	81	48.15	−38.70	−47.78	−0.99	TGAAGCTGCCACATGATCTGA	71	ND	−	72
eun-MIR169	Contig142088	730	39.18	−160.80	−22.03	−0.56	TTATAGGCGATTGGAGGTATG	876	TTAGCTAAAGTCGTCTTGCCCA	6818	8961
eun-MIR172-1	Contig83802	122	47.54	−55.10	−45.16	−0.95	CAGGTGTAGCATCATCAAGAT	36	AGAATCTTGATGATGCTGCAT	495	1076
eun-MIR172-2	Contig113567	92	42.39	−39.70	−43.15	−1.02	GCAGCATCATCAAGATTCACA	12	AGAATCTTGATGATGCTGCAT	495	523
eun-MIR172-3	Contig85928	165	43.64	−71.20	−43.15	−0.99	GTAGCATCATCAAGATTCACA	33	AGAATCTTGATGATGCTGCAT	495	1048
eun-MIR395	Contig114717	88	51.14	−47.50	−53.98	−1.06	TCCCCTAGAGTTCTCCTGAACA	107	ATGAAGTGTTTGGGGGAACTC	1356	1659
eun-MIR396-1	Contig94388	160	42.50	−68.60	−42.88	−1.01	TTCCACGGCTTTCTTGAACTG	217485	GTTCAATAAAGCTGTGGGAAG	2028	223828
eun-MIR396-2	Contig153308	128	42.19	−49.10	−38.36	−0.91	TTCCACAGCTTTCTTGAACTG	23061	GTTCAAGCTAGCTGTGGGAAG	12981	64439
eun-MIR397-1	Contig87345s	126	51.59	−68.80	−54.60	−1.06	TCATTGAGTGCAGCGTTGAT	626	CGGTTTCGACAGCGCTGCACT	59	1052
eun-MIR397-2	Contig87345a	126	51.59	−63.20	−50.16	−0.97	TGCAGCGCTGTCGAAACCGAT	20	TCAACGCTGCACTCAATGATG	273	322
eun-MIR482-1	Contig88445	153	53.90	−94.60	−61.43	−1.14	CATGGGTTGTTTGGTGAGAGG	24202	TCTTGCCAATACCACCCATGCC	70833	100235
eun-MIR482-2	Contig88445	139	53.96	−71.00	−51.08	−0.95	GAAATGGGAGGGTGGGAAAGA	982	TTTCCTATTCCTCCCATTCCAT	3371	5574
eun-MIR482-3	Contig85065	169	50.89	−92.40	−54.67	−1.07	GAGATTCGAGCTACCGGAAGTTGTG	329	TTCCCAAGGCCGCCCATTCCGA	14915	17039
eun-MIR530	Contig18750	183	51.37	−82.30	−44.97	−0.88	TCTGCATTTGCACCTGCACCT	185	AGGTGCGGGTGCAGGTGCAGA	12	280
eun-MIR535-1	Contig68094	102	50.00	−42.60	−41.76	−0.84	TGACAACGAGAGAGAGCACGC	62562	GTGCTCTCTATCGCTGTCATA	4199	76334
eun-MIR535-2	Contig71803	100	49.00	−47.00	−47.00	−0.96	TGACAACGAGAGAGAGCACGC	62562	TGCTCTCTACCGTTGTCATG	116	72263
eun-MIR827	Contig93928	81	45.68	−44.30	−54.69	−1.20	CTTTGTTGATGGCCATCTAATC	27	TTAGATGACCATCAGCGAACA	266	304

MFE: minimal folding free energy (kcal/mol); AMFE: Adjusted MFE; MFEI: minimal folding free energy index; ND: no detected.