Table 4. New putative miRNA precursors identified in E. uniflora.
| miRNA | Precursor miRNA | Mature miRNA | |||||||||
| Contig name | Length | GC % | MFE | AMFE | MFEI | 5p more abundant sequence | Read count | 3p more abundant sequence | Read count | Total reads | |
| eun-nMIR001-1 | Contig164780s | 820 | 54.39 | −451.70 | −55.09 | −1.01 | TCGGCTGTCAATTTCTGGATT | 185919 | ATCCAGAAATTGGCAGCCGTT | 110 | 192103 |
| eun-nMIR001-2 | Contig164780a | 820 | 54.39 | −446.00 | −54.39 | −1.00 | CAATTTCTGGATTTCAGTTCG | 20 | TCGAACTGAAATCCAGAAATT | 2 | 63 |
| eun-nMIR002 | Contig29785 | 173 | 30.06 | −56.70 | −32.77 | −1.09 | CGAAAAATGATTGGTTGTATCGCT | 18 | CGATCTAATCAATCATTTTTCGGG | 2 | 21 |
| eun-nMIR003 | Contig121100 | 110 | 48.18 | −63.20 | −57.45 | −1.19 | TACTCGTTCCGTTGATCCATC | 88 | TGGATCAATAGAACGAGCAGGTGA | 157 | 561 |
| eun-nMIR004-1 | Contig37387s | 104 | 29.81 | −47.10 | −45.29 | −1.52 | TCGTAAATCCACTATATCTCT | 3 | TAGATATAGTGGATTTTCGAT | 9 | 13 |
| eun-nMIR004-2 | Contig37387a | 104 | 29.81 | −46.40 | −44.62 | −1.50 | ND | − | CAGAGATATAGTGGATTTACG | 314 | 385 |
| eun-nMIR005 | Contig143563 | 106 | 34.91 | −21.40 | −20.19 | −0.58 | ND | − | GAGAATGATGAGTTAAATGGA | 12 | 30 |
| eun-nMIR006 | Contig113617 | 113 | 33.63 | −42.70 | −37.79 | −1.12 | TCTCTGTTGATCTGATAAATA | 19 | TTTGTCGGATGAACAGGGAAT | 18 | 55 |
| eun-nMIR007 | Contig116664r | 96 | 46.88 | −55.80 | −58.13 | −1.24 | TAGGGTCAGATCGCTACTTAG | 211 | TAAGTGGTGATCTGACTCTAA | 4446 | 5750 |
| eun-nMIR008 | Contig79716 | 426 | 43.66 | −228.00 | −53.52 | −1.23 | TCGAGCCCCTCCCACAGATTG | 313 | ATCTGTGGAAGAGACTCGACT | 8403 | 13617 |
| eun-nMIR009 | Contig81320 | 1545 | 37.41 | −397.90 | −25.75 | −0.69 | TTCAAGTCTAACAACCTCAGCT | 5774 | TGGAGGTTGTTTGGCTTGAGCT | 1968 | 11577 |
| eun-nMIR010 | Contig84248 | 350 | 48.86 | −143.30 | −40.94 | −0.84 | TGCTGTTCTTCCGTTCACGAAT | 309 | TCGTGAAGGAAGAATGTGCAAT | 6340 | 10307 |
| eun-nMIR011 | Contig164559 | 207 | 51.21 | −98.70 | −47.68 | −0.93 | GCTCGAGGTCAGTTTGTCGCC | 1553 | CGGCAAACTGGACCTCGAGATC | 110 | 2884 |
| eun-nMIR012 | Contig167957 | 164 | 35.98 | −91.80 | −55.98 | −1.56 | CATGTAACAAGTTGGCTGTCA | 126 | TTCATGGACAGCCAGCTTATT | 3 | 243 |
| eun-nMIR013 | Contig87665 | 179 | 56.42 | −84.90 | −47.43 | −0.84 | TGAAGCAGATCAAGAACCCAG | 155 | TCTCGTTCCGCTTCATCTGAA | 2 | 172 |
| eun-nMIR014 | Contig200629r | 87 | 55.17 | −23.40 | −26.90 | −0.49 | ND | − | GCATCACTAGCTTACGCTCTG | 30 | 159 |
| eun-nMIR015 | Contig165886 | 124 | 37.90 | −45.10 | −36.37 | −0.96 | ND | − | CAATGAACGCATTTGCAGGTG | 21 | 22 |
MFE: minimal folding free energy (kcal/mol); AMFE: Adjusted MFE; MFEI: minimal folding free energy index; ND: no detected.