Figure 3. Synergistic activation of HIV-1 promoter by M344 and TNF-α, 5-Aza and prostratin in latently infected cells.

J-Lat clones A7 cells were mock treated or treated with M344 (50 nM), TNF-α (10 ng/ml), 5-Aza (500 nM), prostratin (100 nM), M344/TNF-α, M344/5-Aza or M344/prostratin. The effects of synergistic activation of HIV-1 promoter were determined by quantifying the GFP-positive cells using flow cytometry 72 hours after treatment. Results are presented as fluorescence histograms. Summary of synergistic activation assays are presented as histograms. Data represent the means standard deviations of three independent experiments.