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. 2012 Nov 15;7(11):e48832. doi: 10.1371/journal.pone.0048832

Figure 8. Subcellular localization of p65.

Figure 8

Immunofluorescence analysis of the p65 protein in J-Lat clones A7 cells mock treated or treated with M344, or TNF or TSA for 30 minutes or 2 hours. Subcellular localization of p65 was determined via indirect immunofluorescence employing rabbit polyclonal anti-p65 and goat anti-rabbit antibody coupled to Alexa-555. DAPI staining was used to determine the region of nuclei and to assess gross cell morphology.