Figure 9. M344 induces RelA recruitment to the latent HIV-1 LTR.

J-Lat clones A7 were stimulated with M344 (200 nM) or TNF-α(10 ng/ml) for 4 hours, respectively. Chromatin immunoprecipitation assays were performed using anti-p65 or anti-p50 antibodies or rabbit preimmune IgG and probed for the HIV LTR DNA sequences spanning the κB enhancer or for nonspecific control β-actin. Each ChIP experiment was repeated three times to confirm reproducibility of results and real-time quantitation of the fold change relative to untreated control is shown.