Figure 7. IRAK mediates eNOS activation and inhibitory site phosphorylation in LTA-treated PMEM.

Western blots were generated from PMEM pre-treated for 2 hours in the absence or presence of increasing concentrations of IRAK1/4 inhibitor (IRI) and then co-treated with LTA (10 µg/mL) for 4 hours. (a) The Western blot band densities of phosphorylated serine 1177 of eNOS, its major activation site. (b) The Western blot band densities of phosphorylated threonine 495 of eNOS, a major inhibitory site. (c) The ratios of the band densities in panel (a) over those in panel (b). The units for panels a – c are relative density units (RDU). (d) Representative Western blots. Values represent means ± SEM (N ≥4). * p<0.03 vs. media alone, # p<0.03 vs. LTA alone.