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. 2012 Nov 15;8(11):e1003012. doi: 10.1371/journal.ppat.1003012

Figure 3. LpRalF and RpRalF capping domains associate with different subcellular compartments.

Figure 3

A) and B) Fluorescence microscopy of HeLa cells transfected with YFP-LpRalF192–374. Cells were fixed 24 hours after transfection, then stained with anti-PDI (A) or anti-GM130 (B) antibodies as indicated. Bar = 10 µm. C) Fluorescence microscopy of HeLa cells transfected with YFP-RpRalF189–458 and RFP-PALM. Bar = 5 µm. D) Confocal microscopy shows that RpRalF189–458 localizes at the plasma membrane. E) RpRalF capping domain is sufficient to target plasma membrane. Fluorescence microscopy of HeLa cells transfected with YFP-RpRalF189–359. Bar = 10 µm.