Figure 6. RpRalF inefficiently restores Arf1 recruitment to vacuoles containing Legionella ΔralF.

A) Representation of the constructs used in the complementation experiment. B) HEK293 cells stably expressing Arf1-GFP were infected with different strains of L. pneumophila (wt, ΔralF mutant and ΔralF mutant complemented with M45-LpRalF, M45-LpRalF_E103A, M45-RpRalF_1–342SS or M45-RpRalF_{1–342SSE100A},). Cells were fixed 1 h post-infection, extracellular bacteria were stained in blue, and total bacteria in red. Bar = 1 µm. C) Quantification of Arf1-GFP recruitment to the LCV by the indicated L. pneumophila strains. Represented is the average of 3 experiments where 50 vacuoles were counted. Standard deviations are indicated.