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. 2012 Nov 15;8(11):e1003024. doi: 10.1371/journal.ppat.1003024

Figure 6. 2-AA pretreatment alters activation of the MAPKs and AP-1 in macrophages upon 2-AA stimulation.

Figure 6

Western blotting of cellular extracts with phospho-specific antibodies after 48 h pretreatment with 2-AA (0.8 mM) followed by stimulation with 0.4 mM 2-AA (A) p38 MAPK, (B) JNK1/2 and (C) ERK1/2. One representative experiment (out of three) is shown. Loading was normalized relative to mouse β-actin. A TransAM AP-1 transcription factor assay after 48-h pretreatment with 2-AA (0.8 mM) followed by stimulation with 2-AA, showing the binding of c-Fos (D) and c-Jun (E) to AP-1 promoter. Mean values calculated from three replicate experiments are depicted with SD error bars. (p<0.05, Student's t test).