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. 2012 Nov 15;8(11):e1003032. doi: 10.1371/journal.ppat.1003032

Figure 4. Pns10 tubules on actin-based microvilli of midgut in viruliferous leafhoppers.

Figure 4

At 6-day padp, leafhopper organs were immunolabeled for Pns10 tubules with Pns10-rhodamine (red), for RDV virions with virus-Alexa Fluor 647 (blue), and for actin-based microvilli with FITC-phalloidin (green), then examined with confocal microscopy. Images of anterior midgut (A), middle midgut (B, C) and posterior midgut (D) are shown. Images I in B and C were merged with red fluorescence (Pns10 tubules), blue fluorescence (virus antigens) and green fluorescence (actin). Images I in A and D were merged with red fluorescence (Pns10 tubules), blue fluorescence (virus antigens) and green fluorescence (actin) with background visualized by transmitted light. Images II were single green fluorescence (actin) to show the actin-based microvilli. GL, gut lumen. Mv, microvilli. VM, visceral muscles. Images are representative of multiple experiments with multiple preparations. Bars, 10 µm.