Skip to main content
. 2012 Jul 30;4(5):1033–1036. doi: 10.3892/ol.2012.834

Figure 2.

Figure 2

Effect of KLF4 on Bcl-2/Bax expression and cell proliferation. (A) Real-time RT-PCR analysis of Bcl-2/Bax expression in KLF4-expressing and control vector-only SKOV3 and SNU251 cells. GAPDH mRNA was used as an internal control. Mean values were compared with the value of empty vector-transfected cells to calculate relative amounts of transcripts; (B) MTT cell proliferation assays of KLF4-expressing and vector-only SKOV3 and SNU251 cells from 1 and 2-day cultures. Each bar represents the fold change compared to the value for empty vector-transfected cells cultured for 24 h; (C) Immunoblot analysis of KLF4 protein in transfected cells. β-actin was used as an internal control. EV, empty vector; KLF4, pCMV3xFLAG-KLF4 plasmid (2 μg). Data are the means ± SD of 3 separate experiments. P-values were compared to EVs using the Student’s t-test (*P=0.024; **P=0.014).