Figure 3.

Fatty acid synthase (FASN) expression in osteosarcoma (OS) with and without pulmonary metastasis (x400). Representative images of (a) H&E staining for OS tissues without pulmonary metastasis showing that OS is cell-rich and has significant cellular atypia, ansionucleosi, prominent nucleoli, abundant cytoplasm and a small amount of bone-like matrix; (b) IHC staining for FASN in OS tissues without pulmonary metastasis, showing yellow particle deposition in the cytoplasm with only a small amount of the cells, approximately 10%, colored; (c) H&E staining for OS tissues with pulmonary metastasis showing that OS is cell-rich and has significant cellular atypia, ansionucleosi, prominent nucleoli and abundant cytoplasm; (d) IHC staining for FASN with lung metastasis showing brown particles deposited in the cytoplasm with the majority of cells, approximately 92%, colored. H&E, hematoxylin and eosin; IHC, immunohistochemistry.