Figure 3.

Apoptosis induced by IFNγ in HepG2 cells with excess β-catenin is STAT3-dependent. HepG2 cells were transfected with plasmid expressing active β-catenin. The transfected cells were then treated with or without IFNγ (100 ng/ml) in the presence or absence of (A) STAT1 inhibitor FLUD or (B) STAT3 inhibitor S3I, and active STAT1 and STAT3 levels were determined by flow cytometry, respectively. The transfected cells were treated with or without IFNγ (100 ng/ml) in the presence or absence of (C) FLUD or (D) S3I for 48 h, and apoptosis induction was evaluated by TUNEL assay. ^*P<0.05 compared with corresponding untreated cells. IFN, interferon; FLUD, fludarabine.