Fig. 5.

IFN-γ upregulates the immunogenicity of the B16F10 melanoma line. Flow cytometry was used to measure the density of ai the IFN-γR1 and aii IFN-γR2 chains and b upregulation of the MHC-I H-2D^b allele at the surface of in vitro cultured B16F10 cells, in response to IFN-γ. c The capacity of gp100_25–33-specific pMel T cells to exhibit cytotoxicity toward B16F10 was augmented by preincubating the B16F10 line with 100 IU/ml IFN-γ overnight, prior to co-culture with T cells in ⁵¹Cr-release assays (data compiled from 3 independent experiments)