Figure 4.
Biochemical characterization of 5 selected compounds. (A) EMSA-based analyses of the binding of compounds 1, 5, 8, 9, or 10 to NC’ showing the displacement of FSL-2 from the NC’- FSL-2 complex or the internal control utilizing 10 µM unlabeled SL-2 DNA. (B) Resonant waveguide grating-based analyses of the binding of compounds 1, 5, 8, 9, or 10 to immobilized NC’. Real-time kinetics of compound binding was monitored by peak wavelength value shifts over time.