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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Nature. 2012 Oct 17;491(7424):463–467. doi: 10.1038/nature11522

Figure 1. Effector T cells are recruited to the vagina by topical chemokine treatment.

Figure 1

a, Experimental schematic. Donor gBT-I CD8 T cell recipients were unimmunized (naïve), or immunized either ivag or s.c. with TK- HSV-2. Five days post-infection, s.c. immunized mice was treated vaginally with either the chemokines (pull) or PBS. One day post-pull, the frequency (b) and number (c, d) of donor gBT-I CD8 T cells (b, c) and CD4 T cells (d) were determined in the indicated tissues. Plots are gated on total CD8 T cells and numbers indicate % gBT-I (CD45.1+) (b). Numbers in graphs indicate fold difference in T cell number between s.c. vs. s.c. + pull. Statistical significance was determined by two-tailed unpaired Student’s t-test. Data are pooled from 2–7 independent experiments; n = 6–21 per group. All error bars show standard error (SEM), and *; p = 0.05–0.01, **; p = 0.01 – 0.001, ***; p < 0.001 throughout the manuscript (individual p values are found in Supplementary Information).