Skip to main content
. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Nature. 2012 Oct 17;491(7424):463–467. doi: 10.1038/nature11522

Figure 4. Prime and pull protects mice from lethal genital HSV-2 challenge.

Figure 4

Mice were immunized as shown in Figure 1a and challenged vaginally with a lethal dose of HSV-2 four weeks post-pull. Weight loss (a), disease severity (b), and survival (c) were assessed. d, HSV-2 was titered from vaginal washes collected at the indicated time points. Dashed line indicates limit of detection. ND = none detected. n = 11 (unimmunized), n = 9 (ivag), n = 12 (s.c., s.c. + pull). e, Viral titers were measured in the dorsal root ganglia 6–7 days post-challenge. # × N.D. = number of mice in which no virus was detected. n = 6–11 per group. Statistical significance was measured by two-way ANOVA (a-b, d), log-rank (Mantel-Cox) test (c) or two-tailed unpaired Student’s t-test (e). Data are pooled from 3–5 independent experiments.