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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Neuroendocrinol. 2012 Dec;24(12):1542–1552. doi: 10.1111/j.1365-2826.2012.02362.x

Figure 1.

Figure 1

JunD mRNA expression changes in the SON after 1 and 3 days of dehydration: (A) Fold change after dehydration of JunD mRNA levels were assessed relative to the internal control 18S rRNA using the Quantitect SYBR Green RT-PCR kit (Qiagen). The plots show three representative samples from one animal in each of the groups (control, 24-hours and 72-hours of water deprivation). Total mRNA was extracted from the SON of control, 24-hour and 72-hour water deprived rats (1 rat per total mRNA sample, n=4 for each condition, the PCR were run in triplicate). (B) The fold change of JunD mRNA expression was calculated as follows: Ratio (test/CTR) = 2−ΔΔCT, where ΔΔCT = [CT JunD(dehydrated rat) − CT 18S(dehydrated rat) − CT JunD(control) − CT 18S(control)]. Mean fold change ± S.E.M. *P<0.05 compared to control (Con), one-way ANOVA, bonferroni’s correction.