Skip to main content
. 2012 Oct 16;1(10):e50. doi: 10.1038/mtna.2012.12

Figure 2.

Figure 2

Schematic representation of constructs used in the study. (a) piggyBac transposon constructs were developed to express CMV-driven GFP together with the puromycin resistance gene (pXLBacII_GFPPuro) and either firefly luciferase or human α1 antitrypsin under the control of the liver-specific murine albumin enhancer/human α1 antitrypsin hybrid promoter. The promoter and transgene are flanked by identical 13-bp inverted terminal repeats which are shaded gray. (b) piggyBac transposase constructs. The murine codon-optimized (mPB) hyperactive (iPB7) or inactive (mPBD268L) were inserted into the mammalian expression vector pcDNA3.1. Asterisks indicate sites of amino acid substitutions; black bars indicate catalytic residues D268, D346, and D447.23,24,25 PB, piggyBac transposase; CMV, cytomegalovirus.