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. 2012 Oct 16;1(10):e50. doi: 10.1038/mtna.2012.12

Figure 4.

Figure 4

Hyperactive piggyBac transposase-mediated secreted protein gene transfer persists in vivo. (a) 5 µg pXLBacII-albhaathAAT trasponson was given with varying quantities of pcDNA3.1-iPB7 to create an in vivo dose–response curve using ratios of 5:1(shaded triangle), 2:1(gray square), or 1:1(gray diamond) transposon:transposase. DNA was prepared in 2 ml Lactated Ringer's solution and delivered hydrodynamically to 8- to 10-week-old C57BL/6 mice. Serum hAAT concentrations were measured using ELISA. n = 4–6 for each condition; points indicate means plus standard error. (b) 25 µg pXLBacIIalbhaathAAT transposon was given with varying quantities of pcDNA3.1-iPB7 as described above. (c) Serum hAAT concentrations at 3 months. The transposon:iPB7 ratio is indicated just above the horizontal axis and the transposon dose (5 µg or 25 µg) above the ratios. n = 4–6 mice for each condition; points indicate means plus standard error. * indicates P < 0.01. LR, Lactated Ringer's.