Table II.
1H Chemical shifts of oligosaccharide II (fraction 6.5)
| Unit | Codea | Chemical Shift (δ)
|
|||||
|---|---|---|---|---|---|---|---|
| H-1 | H-2 | H-3 | H-4 | H-5 | H-6 | ||
| Rha | Aα | 5.22 | 3.97 | 4.09 | 3.71 | 3.95 | 1.34 |
| Aβ | 4.95 | 4.06 | 3.86b | 3.60b | 3.52b | 1.35b | |
| α-GalA | B | 5.08, 5.16c | 3.92, 3.97c | 4.13, 4.15c | 4.42 | 4.64 | —d |
| α-Rha | C | 5.27 | 4.13 | 4.09 | 3.67 | 3.85 | 1.30 |
| α-GalA | D | 5.04 | 3.93 | 4.12 | 4.42 | 4.64 | — |
| α-Rha | E | 5.32 | 4.31 | 4.09 | 3.62 | 3.85 | 1.29 |
| us-α-GalA | F | 5.14 | 3.80 | 4.34 | 5.81 | — | — |
| β-Gale | 4.63 | 3.51 | 3.66 | 3.90 | n.d.f | n.d. | |
| ?β-Xylg | 4.48 | 3.29 | 3.55 | 3.78 | 3.37, 4.10 | — | |
a
Backbone sugar units are coded A to F from reducing end to nonreducing end.
b
Tentative assignment (weak signals).
c
Two values are for unit B linked to Aα and Aβ, respectively.
d
−, Not present.
e
Three residues, linked to A, C, and E. Not distinguished, δ values differ by <0.01 ppm.
f
n.d., Not determined.
g
Detected in fraction 6 but not part of oligosaccharide II (see text).