Table 1. Influence of β2-microglobulin on HLA-B7 expression.

Treatment	VM92 cells		293 Cells
	% HLA-B7 positive	Mean FI	% HLA-B7 positive	Mean FI
None	3%	39	63%	24
VCL-1004	13%	31	93%	58
VCL-1005	60%	123	98%	143

VM92 cells (murine origin, HLA-B7 negative and β2M defective) or 293 cells (human origin, HLA-B7 and β2M positive) were transfected in vitro with either VCL-1004 (encoding HLA-B7) or VCL-1005 (encoding both HLA-B7 and β2M), delivered as 5 μg plasmid formulated with dimyristyloxypropyl-3-dimethyl-hydroxyethyl ammonium/dioleoyl phosphatidylethanolamine; cells that received no treatment were used as a control. Flow cytometry was then performed 48 h later to determine the percentage of cells expressing HLA-B7 (as detected by the monoclonal antibody BB7.1) and the mean fluorescence intensity (FI, in arbitrary units) of those cells.