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POMC protein detection by western blotting in whole cell lysates and in culture media. Lysates panel: Blot of whole-cell lysates from WTPOMC (WT), A15G-POMC (A15G) and non-transfected (NT) β-TC3 cells with and without forskolin/IBMX stimulation (+S/−S). Media panel: Blot of culture media collected from WT, A15G-transfected and NT β-TC3 cells with and without forskolin/IBMX stimulation (+S/−S).
