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. 2012 Nov 16;2:867. doi: 10.1038/srep00867

Figure 4. Sensitivity and specificity of the tetrahedra-based EMRS with poly-HRP80 amplification.

Figure 4

(A) Logarithmic plot of amperometric current vs miR-21 concentration for tetrahedra-based EMRS with poly-HRP amplification. Insert shows the low concentration range of miR-21. Corresponding amperometric curves are shown in Fig. S3. Error bars represent standard deviations for measurements taken from at least three independent experiments. (B) Effect of hybridization efficiency by tuning the ratio of TS and TSP while maintaining the total concentration of DNA tetrahedral in the assembly solution (1 μM). (C) Discrimination of let 7 family members, miR-21 used as negative control (all of 1 pM), * P < 0.05 significantly different from the let-7d, and (D) Pre-/mature miRNA discrimination (of 10 pM), * P < 0.05 significantly different from the pre-miR-31 and blank. Error bars represent standard deviations for measurements taken from at least three independent experiments.